Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3325965..3326619 Replicon chromosome
Accession NZ_AP027217
Organism Escherichia coli strain JNE170426

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag QUE10_RS16230 Protein ID WP_000244772.1
Coordinates 3325965..3326372 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE10_RS16235 Protein ID WP_000354046.1
Coordinates 3326353..3326619 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE10_RS16210 (3321922) 3321922..3323655 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
QUE10_RS16215 (3323661) 3323661..3324371 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE10_RS16220 (3324396) 3324396..3325292 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE10_RS16225 (3325404) 3325404..3325925 + 522 WP_001055874.1 flavodoxin FldB -
QUE10_RS16230 (3325965) 3325965..3326372 - 408 WP_000244772.1 protein YgfX Toxin
QUE10_RS16235 (3326353) 3326353..3326619 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE10_RS16240 (3326862) 3326862..3327842 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QUE10_RS16245 (3327919) 3327919..3328578 - 660 WP_000250274.1 hemolysin III family protein -
QUE10_RS16250 (3328742) 3328742..3329053 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
QUE10_RS16255 (3329098) 3329098..3330531 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
QUE10_RS16260 (3330588) 3330588..3331331 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T43548 WP_000244772.1 NZ_AP027217:c3326372-3325965 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43548 NZ_AP027217:c3326372-3325965 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43548 WP_000354046.1 NZ_AP027217:c3326619-3326353 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43548 NZ_AP027217:c3326619-3326353 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References