Detailed information of TA system TA043546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2862259..2862884
• Replicon: chromosome
• Accession: NZ_AP027217
• Organism: Escherichia coli strain JNE170426

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8S7SZU1
• Locus tag: QUE10_RS14055
• Protein ID: WP_000911327.1
• Coordinates: 2862259..2862657 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QUE10_RS14060
• Protein ID: WP_000450524.1
• Coordinates: 2862657..2862884 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE10_RS14035 (2858137)	2858137..2858337	+	201	WP_000383836.1	YpfN family protein	-
QUE10_RS14040 (2858447)	2858447..2859145	-	699	WP_000679823.1	esterase	-
QUE10_RS14045 (2859219)	2859219..2861234	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
QUE10_RS14050 (2861249)	2861249..2862112	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QUE10_RS14055 (2862259)	2862259..2862657	-	399	WP_000911327.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUE10_RS14060 (2862657)	2862657..2862884	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QUE10_RS14065 (2863039)	2863039..2863752	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QUE10_RS14070 (2863965)	2863965..2864999	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QUE10_RS14075 (2865016)	2865016..2865894	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QUE10_RS14080 (2866040)	2866040..2866612	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QUE10_RS14085 (2866612)	2866612..2867082	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43546 WP_000911327.1 NZ_AP027217:c2862657-2862259 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMEIIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43546 NZ_AP027217:c2862657-2862259 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGATTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43546 WP_000450524.1 NZ_AP027217:c2862884-2862657 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43546 NZ_AP027217:c2862884-2862657 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33