Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 526115..526733 Replicon chromosome
Accession NZ_AP027217
Organism Escherichia coli strain JNE170426

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE10_RS02415 Protein ID WP_001291435.1
Coordinates 526115..526333 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE10_RS02420 Protein ID WP_000344800.1
Coordinates 526359..526733 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE10_RS02380 (521404) 521404..521976 + 573 WP_000779826.1 YbaY family lipoprotein -
QUE10_RS02385 (522007) 522007..522318 - 312 WP_000409911.1 MGMT family protein -
QUE10_RS02395 (522697) 522697..523050 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE10_RS02400 (523092) 523092..524642 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE10_RS02405 (524806) 524806..525276 - 471 WP_000136192.1 YlaC family protein -
QUE10_RS02410 (525392) 525392..525943 - 552 WP_000102564.1 maltose O-acetyltransferase -
QUE10_RS02415 (526115) 526115..526333 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE10_RS02420 (526359) 526359..526733 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE10_RS02425 (527279) 527279..530428 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QUE10_RS02430 (530451) 530451..531644 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T43533 WP_001291435.1 NZ_AP027217:c526333-526115 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T43533 NZ_AP027217:c526333-526115 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT43533 WP_000344800.1 NZ_AP027217:c526733-526359 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT43533 NZ_AP027217:c526733-526359 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References