Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pasABC/RelE-HTH
Location 435..909 Replicon plasmid pJNE131328-2
Accession NZ_AP027214
Organism Escherichia coli strain JNE131328

Toxin (Protein)


Gene name pasB Uniprot ID A0A748ATC0
Locus tag QUE42_RS25515 Protein ID WP_000262325.1
Coordinates 643..909 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID A0A7U2VUZ4
Locus tag QUE42_RS25510 Protein ID WP_000879770.1
Coordinates 435..659 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE42_RS25505 (JNE131328_p20010) 69..281 - 213 WP_000350688.1 hypothetical protein -
QUE42_RS25510 (JNE131328_p20020) 435..659 + 225 WP_000879770.1 DUF6290 family protein Antitoxin
QUE42_RS25515 (JNE131328_p20030) 643..909 + 267 WP_000262325.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUE42_RS25520 (JNE131328_p20040) 968..1390 - 423 WP_032319274.1 Exc2 family lipoprotein -
QUE42_RS25525 (JNE131328_p20050) 1512..1790 + 279 WP_000710675.1 hypothetical protein -
QUE42_RS25530 (JNE131328_p20060) 1782..2072 - 291 WP_024200900.1 hypothetical protein -
QUE42_RS25535 (JNE131328_p20070) 2317..3789 + 1473 WP_000362089.1 colicin-like pore-forming protein -
QUE42_RS25540 3786..4076 - 291 WP_011264161.1 colicin K immunity protein -
QUE42_RS25545 4106..4237 + 132 WP_077521118.1 colicin release lysis protein -
QUE42_RS25550 (JNE131328_p20090) 5246..5437 - 192 WP_000165980.1 Rop family plasmid primer RNA-binding protein -
QUE42_RS25555 (JNE131328_p20100) 5505..5828 + 324 WP_001444404.1 MobC family plasmid mobilization relaxosome protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..15094 15094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-87)

Antitoxin

Relaxosome protein TraY

(4-45)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10455.13 Da        Isoelectric Point: 9.8720

>T43530 WP_000262325.1 NZ_AP027214:643-909 [Escherichia coli]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSGRPLKGELGEFWRYRVGDYRILCEIRDDELIILVATI
GHRREVYD

Download         Length: 267 bp

>T43530 NZ_AP027214:643-909 [Escherichia coli]
TTGGTCTGGACGATTAATTATTCCGATCGGGCGCTCAAATCGCTACGTAAGATGGACAAGCAGAACGCGCGACGGATTGT
GGATTTTATGGATTTACGTATTGCAGTTGCAGCCGATCCCCGTAAATCAGGGAGGCCACTGAAGGGGGAGTTGGGAGAAT
TCTGGCGTTATCGTGTGGGTGATTATCGCATTCTGTGTGAAATCAGGGATGACGAACTGATTATTCTTGTGGCCACCATC
GGCCATCGACGTGAAGTCTACGACTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8369.33 Da        Isoelectric Point: 4.8552

>AT43530 WP_000879770.1 NZ_AP027214:435-659 [Escherichia coli]
MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Download         Length: 225 bp

>AT43530 NZ_AP027214:435-659 [Escherichia coli]
ATGCTGGCTATTCGGTTGTCTGATGAAATTGAGTCCCGTCTGGACTCGCTGGCGAAGAAAACCGGGAGGACAAAGACGTT
CTATGCCCGGGAAGCGATACTGGCGCACCTGGAAGATCTGGAAGATTATTATCTTTCAGCGGAAACAGCAGCACGTGTTC
GCCGTGGTGATGAGGCTGTGCATTCGTCTGAGGACGTAAGGAAGTCTCTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A748ATC0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U2VUZ4

References