Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 155566..156091 Replicon plasmid pJNE131328-1
Accession NZ_AP027213
Organism Escherichia coli strain JNE131328

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag QUE42_RS25465 Protein ID WP_001159871.1
Coordinates 155566..155871 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag QUE42_RS25470 Protein ID WP_000813639.1
Coordinates 155873..156091 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE42_RS25435 151561..151734 + 174 Protein_151 DUF4113 domain-containing protein -
QUE42_RS25440 (JNE131328_p11430) 151736..152152 - 417 WP_001278809.1 plasmid partitioning/stability family protein -
QUE42_RS25445 (JNE131328_p11440) 152145..153125 - 981 WP_000688508.1 plasmid segregation protein ParM -
QUE42_RS25450 (JNE131328_p11450) 153540..153848 - 309 WP_000030199.1 hypothetical protein -
QUE42_RS25455 (JNE131328_p11460) 153935..154579 - 645 WP_001144036.1 ParA family protein -
QUE42_RS25460 (JNE131328_p11470) 154759..155565 - 807 WP_000016966.1 site-specific integrase -
QUE42_RS25465 (JNE131328_p11480) 155566..155871 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
QUE42_RS25470 (JNE131328_p11490) 155873..156091 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
QUE42_RS25475 (JNE131328_p11500) 156687..156947 + 261 WP_000343100.1 hypothetical protein -
QUE42_RS25480 (JNE131328_p11510) 156944..157534 + 591 WP_000194569.1 hypothetical protein -
QUE42_RS25485 (JNE131328_p11520) 157552..157899 - 348 WP_000142426.1 DUF6404 family protein -
QUE42_RS25490 (JNE131328_p11530) 158369..158689 - 321 WP_001365556.1 colicin-like pore-forming protein -
QUE42_RS25495 (JNE131328_p11540) 158799..159530 - 732 WP_000850413.1 conjugal transfer complement resistance protein TraT -
QUE42_RS25500 159807..159959 - 153 Protein_164 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..160700 160700


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T43529 WP_001159871.1 NZ_AP027213:c155871-155566 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T43529 NZ_AP027213:c155871-155566 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT43529 WP_000813639.1 NZ_AP027213:c156091-155873 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT43529 NZ_AP027213:c156091-155873 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References