Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4850293..4850888 Replicon chromosome
Accession NZ_AP027212
Organism Escherichia coli strain JNE131328

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag QUE42_RS23865 Protein ID WP_000239579.1
Coordinates 4850538..4850888 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag QUE42_RS23860 Protein ID WP_001223208.1
Coordinates 4850293..4850544 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE42_RS23850 (4845957) 4845957..4849736 + 3780 WP_000060901.1 autotransporter assembly complex protein TamB -
QUE42_RS23855 (4849739) 4849739..4850080 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
QUE42_RS23860 (4850293) 4850293..4850544 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
QUE42_RS23865 (4850538) 4850538..4850888 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
QUE42_RS23870 (4850968) 4850968..4851498 - 531 WP_000055072.1 inorganic diphosphatase -
QUE42_RS23875 (4851808) 4851808..4852764 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
QUE42_RS23880 (4853074) 4853074..4854576 + 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
QUE42_RS23885 (4854590) 4854590..4855612 + 1023 WP_001339490.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T43528 WP_000239579.1 NZ_AP027212:4850538-4850888 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T43528 NZ_AP027212:4850538-4850888 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT43528 WP_001223208.1 NZ_AP027212:4850293-4850544 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT43528 NZ_AP027212:4850293-4850544 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References