Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4746605..4747102 Replicon chromosome
Accession NZ_AP027212
Organism Escherichia coli strain JNE131328

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag QUE42_RS23345 Protein ID WP_001173344.1
Coordinates 4746929..4747102 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE42_RS23340 Protein ID WP_000398619.1
Coordinates 4746605..4746901 (+) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE42_RS23320 (4743336) 4743336..4744055 - 720 WP_000611288.1 two-component system response regulator DcuR -
QUE42_RS23325 (4744052) 4744052..4745683 - 1632 WP_001216461.1 sensor histidine kinase -
QUE42_RS23330 (4745864) 4745864..4746094 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QUE42_RS23335 (4746106) 4746106..4746378 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QUE42_RS23340 (4746605) 4746605..4746901 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE42_RS23345 (4746929) 4746929..4747102 + 174 WP_001173344.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE42_RS23350 (4747221) 4747221..4748738 - 1518 WP_001295074.1 lysine--tRNA ligase -
QUE42_RS23355 (4748975) 4748975..4750432 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-57)

Antitoxin

(5-94)


Sequences


Toxin        


Download         Length: 58 a.a.        Molecular weight: 6583.12 Da        Isoelectric Point: 10.2288

>T43527 WP_001173344.1 NZ_AP027212:4746929-4747102 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSVFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43527 NZ_AP027212:4746929-4747102 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGTATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43527 WP_000398619.1 NZ_AP027212:4746605-4746901 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43527 NZ_AP027212:4746605-4746901 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References