Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3970790..3971376 Replicon chromosome
Accession NZ_AP027212
Organism Escherichia coli strain JNE131328

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag QUE42_RS19750 Protein ID WP_042014162.1
Coordinates 3971146..3971376 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QUE42_RS19745 Protein ID WP_000593555.1
Coordinates 3970790..3971149 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE42_RS19720 (3966922) 3966922..3967866 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
QUE42_RS19725 (3967863) 3967863..3968696 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
QUE42_RS19730 (3968696) 3968696..3969460 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
QUE42_RS19735 (3969457) 3969457..3970263 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
QUE42_RS19740 (3970269) 3970269..3970670 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
QUE42_RS19745 (3970790) 3970790..3971149 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QUE42_RS19750 (3971146) 3971146..3971376 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
QUE42_RS19755 (3971480) 3971480..3972604 - 1125 WP_001314210.1 ABC transporter permease -
QUE42_RS19760 (3972604) 3972604..3975339 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T43525 WP_042014162.1 NZ_AP027212:c3971376-3971146 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T43525 NZ_AP027212:c3971376-3971146 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT43525 WP_000593555.1 NZ_AP027212:c3971149-3970790 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT43525 NZ_AP027212:c3971149-3970790 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References