Detailed information of TA system TA043519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2854346..2854971
• Replicon: chromosome
• Accession: NZ_AP027212
• Organism: Escherichia coli strain JNE131328

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QUE42_RS14240
• Protein ID: WP_000911330.1
• Coordinates: 2854346..2854744 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QUE42_RS14245
• Protein ID: WP_000450524.1
• Coordinates: 2854744..2854971 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE42_RS14220 (2850224)	2850224..2850424	+	201	WP_000383836.1	YpfN family protein	-
QUE42_RS14225 (2850534)	2850534..2851232	-	699	WP_000679807.1	esterase	-
QUE42_RS14230 (2851306)	2851306..2853321	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
QUE42_RS14235 (2853336)	2853336..2854199	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QUE42_RS14240 (2854346)	2854346..2854744	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUE42_RS14245 (2854744)	2854744..2854971	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QUE42_RS14250 (2855125)	2855125..2855838	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QUE42_RS14255 (2856051)	2856051..2857085	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
QUE42_RS14260 (2857102)	2857102..2857980	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QUE42_RS14265 (2858126)	2858126..2858698	+	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
QUE42_RS14270 (2858698)	2858698..2859168	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43519 WP_000911330.1 NZ_AP027212:c2854744-2854346 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43519 NZ_AP027212:c2854744-2854346 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43519 WP_000450524.1 NZ_AP027212:c2854971-2854744 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43519 NZ_AP027212:c2854971-2854744 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33