Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 496466..497084 Replicon chromosome
Accession NZ_AP027212
Organism Escherichia coli strain JNE131328

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE42_RS02270 Protein ID WP_001291435.1
Coordinates 496466..496684 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE42_RS02275 Protein ID WP_000344800.1
Coordinates 496710..497084 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE42_RS02235 (491755) 491755..492327 + 573 WP_000779831.1 YbaY family lipoprotein -
QUE42_RS02240 (492358) 492358..492669 - 312 WP_000409911.1 MGMT family protein -
QUE42_RS02250 (493048) 493048..493401 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE42_RS02255 (493443) 493443..494993 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE42_RS02260 (495157) 495157..495627 - 471 WP_000136192.1 YlaC family protein -
QUE42_RS02265 (495743) 495743..496294 - 552 WP_000102568.1 maltose O-acetyltransferase -
QUE42_RS02270 (496466) 496466..496684 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE42_RS02275 (496710) 496710..497084 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE42_RS02280 (497630) 497630..500779 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QUE42_RS02285 (500802) 500802..501995 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T43507 WP_001291435.1 NZ_AP027212:c496684-496466 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T43507 NZ_AP027212:c496684-496466 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT43507 WP_000344800.1 NZ_AP027212:c497084-496710 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT43507 NZ_AP027212:c497084-496710 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References