Detailed information of TA system TA043506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 462158..462995
• Replicon: chromosome
• Accession: NZ_AP027212
• Organism: Escherichia coli strain JNE131328

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QUE42_RS02100
• Protein ID: WP_000227784.1
• Coordinates: 462158..462700 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QUE42_RS02105
• Protein ID: WP_001297137.1
• Coordinates: 462684..462995 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE42_RS02080 (457697)	457697..458608	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
QUE42_RS02085 (458776)	458776..459267	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUE42_RS02090 (459395)	459395..460759	-	1365	WP_001000978.1	MFS transporter	-
QUE42_RS02095 (461167)	461167..462102	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
QUE42_RS02100 (462158)	462158..462700	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QUE42_RS02105 (462684)	462684..462995	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QUE42_RS02110 (463180)	463180..464070	-	891	WP_000971336.1	heme o synthase	-
QUE42_RS02115 (464082)	464082..464411	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUE42_RS02120 (464411)	464411..465025	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QUE42_RS02125 (465015)	465015..467006	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUE42_RS02130 (467028)	467028..467975	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43506 WP_000227784.1 NZ_AP027212:c462700-462158 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43506 NZ_AP027212:c462700-462158 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43506 WP_001297137.1 NZ_AP027212:c462995-462684 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43506 NZ_AP027212:c462995-462684 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3