Detailed information of TA system TA043504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 161498..162023
• Replicon: plasmid pJNE082640-1
• Accession: NZ_AP027207
• Organism: Escherichia coli strain JNE082640

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: QUE04_RS24805
• Protein ID: WP_001159871.1
• Coordinates: 161498..161803 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: QUE04_RS24810
• Protein ID: WP_000813639.1
• Coordinates: 161805..162023 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE04_RS24775 (JNE082640_p11660)	157493..157666	+	174	Protein_160	DUF4113 domain-containing protein	-
QUE04_RS24780 (JNE082640_p11670)	157668..158084	-	417	WP_001278809.1	plasmid partitioning/stability family protein	-
QUE04_RS24785 (JNE082640_p11680)	158077..159057	-	981	WP_000688508.1	plasmid segregation protein ParM	-
QUE04_RS24790 (JNE082640_p11690)	159472..159780	-	309	WP_000030199.1	hypothetical protein	-
QUE04_RS24795 (JNE082640_p11700)	159867..160511	-	645	WP_001144036.1	ParA family protein	-
QUE04_RS24800 (JNE082640_p11710)	160691..161497	-	807	WP_000016966.1	site-specific integrase	-
QUE04_RS24805 (JNE082640_p11720)	161498..161803	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
QUE04_RS24810 (JNE082640_p11730)	161805..162023	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
QUE04_RS24815 (JNE082640_p11740)	162619..162879	+	261	WP_000343100.1	hypothetical protein	-
QUE04_RS24820 (JNE082640_p11750)	162876..163466	+	591	WP_000194569.1	hypothetical protein	-
QUE04_RS24825 (JNE082640_p11760)	163484..163831	-	348	WP_000142427.1	DUF6404 family protein	-
QUE04_RS24830 (JNE082640_p11770)	164314..164685	-	372	WP_001373114.1	colicin-like pore-forming protein	-
QUE04_RS24835 (JNE082640_p11780)	164745..165476	-	732	WP_000850414.1	conjugal transfer complement resistance protein TraT	-
QUE04_RS24840	165748..165858	-	111	Protein_173	IS481 family transposase	-
QUE04_RS24845	165862..166079	-	218	Protein_174	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..166641	166641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T43504 WP_001159871.1 NZ_AP027207:c161803-161498 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T43504 NZ_AP027207:c161803-161498 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATCAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT43504 WP_000813639.1 NZ_AP027207:c162023-161805 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT43504 NZ_AP027207:c162023-161805 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A7U9DIR5