Detailed information of TA system TA043502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4468268..4469105
• Replicon: chromosome
• Accession: NZ_AP027206
• Organism: Escherichia coli strain JNE082640

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QUE04_RS21895
• Protein ID: WP_000227784.1
• Coordinates: 4468563..4469105 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A3T7ZCX8
• Locus tag: QUE04_RS21890
• Protein ID: WP_001442339.1
• Coordinates: 4468268..4468579 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE04_RS21865 (4463288)	4463288..4464235	+	948	WP_001239444.1	cytochrome o ubiquinol oxidase subunit II	-
QUE04_RS21870 (4464257)	4464257..4466248	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUE04_RS21875 (4466238)	4466238..4466852	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QUE04_RS21880 (4466852)	4466852..4467181	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUE04_RS21885 (4467193)	4467193..4468083	+	891	WP_000971336.1	heme o synthase	-
QUE04_RS21890 (4468268)	4468268..4468579	+	312	WP_001442339.1	DUF1778 domain-containing protein	Antitoxin
QUE04_RS21895 (4468563)	4468563..4469105	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QUE04_RS21900 (4469161)	4469161..4470096	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
QUE04_RS21905 (4470504)	4470504..4471868	+	1365	WP_001000975.1	MFS transporter	-
QUE04_RS21910 (4471996)	4471996..4472487	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUE04_RS21915 (4472655)	4472655..4473566	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43502 WP_000227784.1 NZ_AP027206:4468563-4469105 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43502 NZ_AP027206:4468563-4469105 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43502 WP_001442339.1 NZ_AP027206:4468268-4468579 [Escherichia coli]
MPGKTATLPDVDKTLKNARVEIKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43502 NZ_AP027206:4468268-4468579 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAAATCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A3T7ZCX8