Detailed information of TA system TA043500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3250323..3250961
• Replicon: chromosome
• Accession: NZ_AP027206
• Organism: Escherichia coli strain JNE082640

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QUE04_RS15565
• Protein ID: WP_000813794.1
• Coordinates: 3250785..3250961 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QUE04_RS15560
• Protein ID: WP_001270286.1
• Coordinates: 3250323..3250739 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE04_RS15540 (3245476)	3245476..3246417	-	942	WP_001251319.1	ABC transporter permease	-
QUE04_RS15545 (3246418)	3246418..3247431	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
QUE04_RS15550 (3247449)	3247449..3248593	-	1145	Protein_3024	ABC transporter substrate-binding protein	-
QUE04_RS15555 (3248835)	3248835..3250244	-	1410	WP_047081861.1	PLP-dependent aminotransferase family protein	-
QUE04_RS15560 (3250323)	3250323..3250739	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QUE04_RS15565 (3250785)	3250785..3250961	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QUE04_RS15570 (3251183)	3251183..3251413	+	231	WP_000494244.1	YncJ family protein	-
QUE04_RS15575 (3251505)	3251505..3253466	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QUE04_RS15580 (3253539)	3253539..3254075	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
QUE04_RS15585 (3254128)	3254128..3255342	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3255382..3256530	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43500 WP_000813794.1 NZ_AP027206:c3250961-3250785 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43500 NZ_AP027206:c3250961-3250785 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT43500 WP_001270286.1 NZ_AP027206:c3250739-3250323 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT43500 NZ_AP027206:c3250739-3250323 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB