Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2141956..2142581 Replicon chromosome
Accession NZ_AP027206
Organism Escherichia coli strain JNE082640

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag QUE04_RS10255 Protein ID WP_000911329.1
Coordinates 2142183..2142581 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag QUE04_RS10250 Protein ID WP_000450524.1
Coordinates 2141956..2142183 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE04_RS10225 (2137758) 2137758..2138228 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
QUE04_RS10230 (2138228) 2138228..2138800 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
QUE04_RS10235 (2138946) 2138946..2139824 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
QUE04_RS10240 (2139841) 2139841..2140875 + 1035 WP_047081704.1 outer membrane protein assembly factor BamC -
QUE04_RS10245 (2141088) 2141088..2141801 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
QUE04_RS10250 (2141956) 2141956..2142183 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
QUE04_RS10255 (2142183) 2142183..2142581 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
QUE04_RS10260 (2142728) 2142728..2143591 + 864 WP_047081705.1 neutral zinc metallopeptidase -
QUE04_RS10265 (2143606) 2143606..2145621 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
QUE04_RS10270 (2145695) 2145695..2146393 + 699 WP_000679823.1 esterase -
QUE04_RS10275 (2146503) 2146503..2146703 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T43489 WP_000911329.1 NZ_AP027206:2142183-2142581 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T43489 NZ_AP027206:2142183-2142581 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT43489 WP_000450524.1 NZ_AP027206:2141956-2142183 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT43489 NZ_AP027206:2141956-2142183 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References