Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1012495..1013126 Replicon chromosome
Accession NZ_AP027206
Organism Escherichia coli strain JNE082640

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag QUE04_RS04745 Protein ID WP_001259388.1
Coordinates 1012495..1012770 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QUE04_RS04750 Protein ID WP_000593555.1
Coordinates 1012767..1013126 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE04_RS04730 (1007499) 1007499..1008566 + 1068 WP_029488497.1 HlyD family efflux transporter periplasmic adaptor subunit -
QUE04_RS04735 (1008563) 1008563..1011298 + 2736 WP_137462578.1 ribosome-associated ATPase/putative transporter RbbA -
QUE04_RS04740 (1011298) 1011298..1012422 + 1125 WP_001314210.1 ABC transporter permease -
QUE04_RS04745 (1012495) 1012495..1012770 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
QUE04_RS04750 (1012767) 1012767..1013126 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QUE04_RS04755 (1013246) 1013246..1013647 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
QUE04_RS04760 (1013653) 1013653..1014459 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
QUE04_RS04765 (1014456) 1014456..1015220 - 765 WP_047081515.1 nickel import ATP-binding protein NikD -
QUE04_RS04770 (1015220) 1015220..1016053 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
QUE04_RS04775 (1016050) 1016050..1016994 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T43484 WP_001259388.1 NZ_AP027206:1012495-1012770 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T43484 NZ_AP027206:1012495-1012770 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT43484 WP_000593555.1 NZ_AP027206:1012767-1013126 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT43484 NZ_AP027206:1012767-1013126 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References