Detailed information of TA system TA043472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 7088..7581
• Replicon: plasmid pO26_02E060_2
• Accession: NZ_AP027199
• Organism: Escherichia coli strain 02E060

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: QMG97_RS29800
• Protein ID: WP_001230709.1
• Coordinates: 7088..7342 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: QMG97_RS29805
• Protein ID: WP_000151456.1
• Coordinates: 7339..7581 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG97_RS29765 (F02E060_p2_02)	2134..2436	-	303	WP_001096511.1	hypothetical protein	-
QMG97_RS29770 (F02E060_p2_03)	2526..3185	-	660	WP_000516232.1	division plane positioning ATPase MipZ	-
QMG97_RS29775 (F02E060_p2_04)	3738..4061	+	324	WP_000432873.1	hypothetical protein	-
QMG97_RS29780 (F02E060_p2_06)	4415..4759	+	345	WP_001146936.1	type II toxin-antitoxin system RelE/ParE family toxin	-
QMG97_RS29785 (F02E060_p2_07)	4746..5096	+	351	WP_000127482.1	addiction module antitoxin	-
QMG97_RS29790 (F02E060_p2_08)	5930..6445	+	516	WP_001025393.1	J domain-containing protein	-
QMG97_RS29795 (F02E060_p2_09)	6549..7091	+	543	WP_001083909.1	hypothetical protein	-
QMG97_RS29800 (F02E060_p2_10)	7088..7342	+	255	WP_001230709.1	hypothetical protein	Toxin
QMG97_RS29805 (F02E060_p2_11)	7339..7581	+	243	WP_000151456.1	hypothetical protein	Antitoxin
QMG97_RS29810 (F02E060_p2_12)	8152..8445	+	294	WP_000673431.1	hypothetical protein	-
QMG97_RS29815 (F02E060_p2_14)	8750..9037	+	288	WP_000606467.1	hypothetical protein	-
QMG97_RS29820 (F02E060_p2_15)	9465..9698	+	234	WP_000093745.1	hypothetical protein	-
QMG97_RS29825 (F02E060_p2_16)	9774..10076	+	303	WP_000379301.1	hypothetical protein	-
QMG97_RS29830	10348..10497	+	150	WP_000903193.1	hypothetical protein	-
QMG97_RS29835 (F02E060_p2_17)	10608..11429	+	822	WP_001234372.1	DUF932 domain-containing protein	-
QMG97_RS29840 (F02E060_p2_18)	11726..12328	-	603	WP_072205450.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..49687	49687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T43472 WP_001230709.1 NZ_AP027199:7088-7342 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T43472 NZ_AP027199:7088-7342 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT43472 WP_000151456.1 NZ_AP027199:7339-7581 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT43472 NZ_AP027199:7339-7581 [Escherichia coli]
ATGACTATCGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5