Detailed information of TA system TA043460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3468776..3469401
• Replicon: chromosome
• Accession: NZ_AP027197
• Organism: Escherichia coli strain 02E060

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QMG97_RS18265
• Protein ID: WP_000911330.1
• Coordinates: 3468776..3469174 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QMG97_RS18270
• Protein ID: WP_000450524.1
• Coordinates: 3469174..3469401 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG97_RS18245 (3464654)	3464654..3464854	+	201	WP_000383836.1	YpfN family protein	-
QMG97_RS18250 (3464964)	3464964..3465662	-	699	WP_000679812.1	esterase	-
QMG97_RS18255 (3465736)	3465736..3467751	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
QMG97_RS18260 (3467766)	3467766..3468629	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QMG97_RS18265 (3468776)	3468776..3469174	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG97_RS18270 (3469174)	3469174..3469401	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QMG97_RS18275 (3469555)	3469555..3470268	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QMG97_RS18280 (3470481)	3470481..3471515	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QMG97_RS18285 (3471532)	3471532..3472410	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QMG97_RS18290 (3472556)	3472556..3473128	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QMG97_RS18295 (3473128)	3473128..3473598	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43460 WP_000911330.1 NZ_AP027197:c3469174-3468776 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43460 NZ_AP027197:c3469174-3468776 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43460 WP_000450524.1 NZ_AP027197:c3469401-3469174 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43460 NZ_AP027197:c3469401-3469174 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33