Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2545170..2545641 Replicon chromosome
Accession NZ_AP027197
Organism Escherichia coli strain 02E060

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag QMG97_RS13455 Protein ID WP_001448501.1
Coordinates 2545363..2545641 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag QMG97_RS13450 Protein ID WP_001302048.1
Coordinates 2545170..2545361 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG97_RS13400 (2540248) 2540248..2540466 - 219 WP_000683609.1 DUF4014 family protein -
QMG97_RS13405 (2540594) 2540594..2540905 - 312 WP_001289673.1 hypothetical protein -
QMG97_RS13410 (2540898) 2540898..2541125 - 228 WP_000699809.1 hypothetical protein -
QMG97_RS13415 (2541122) 2541122..2541403 - 282 WP_000603384.1 DNA-binding protein -
QMG97_RS13420 (2541436) 2541436..2542197 - 762 WP_000450872.1 DUF1627 domain-containing protein -
QMG97_RS13425 (2542223) 2542223..2542964 - 742 Protein_2635 ATP-binding protein -
QMG97_RS13430 (2542971) 2542971..2543933 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
QMG97_RS13435 (2543956) 2543956..2544381 - 426 WP_000693943.1 toxin YdaT family protein -
QMG97_RS13440 (2544378) 2544378..2544680 - 303 WP_000172738.1 transcriptional regulator -
QMG97_RS13445 (2544778) 2544778..2545149 + 372 WP_001169687.1 hypothetical protein -
QMG97_RS13450 (2545170) 2545170..2545361 + 192 WP_001302048.1 hypothetical protein Antitoxin
QMG97_RS13455 (2545363) 2545363..2545641 + 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QMG97_RS13460 (2545928) 2545928..2546080 + 153 WP_001427316.1 DUF1391 family protein -
QMG97_RS13465 (2546501) 2546501..2546722 + 222 WP_000560218.1 killing protein KilR -
QMG97_RS13470 (2546722) 2546722..2546892 + 171 WP_001427414.1 YdaE family protein -
QMG97_RS13475 (2546966) 2546966..2547241 + 276 WP_001307773.1 hypothetical protein -
QMG97_RS13480 (2547340) 2547340..2549991 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -
QMG97_RS13485 (2549984) 2549984..2550388 + 405 Protein_2647 recombinase RecT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 2495152..2559788 64636


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T43449 WP_001448501.1 NZ_AP027197:2545363-2545641 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T43449 NZ_AP027197:2545363-2545641 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT43449 WP_001302048.1 NZ_AP027197:2545170-2545361 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT43449 NZ_AP027197:2545170-2545361 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References