Detailed information of TA system TA043449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2545170..2545641
• Replicon: chromosome
• Accession: NZ_AP027197
• Organism: Escherichia coli strain 02E060

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A1Y2Y8G6
• Locus tag: QMG97_RS13455
• Protein ID: WP_001448501.1
• Coordinates: 2545363..2545641 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: QMG97_RS13450
• Protein ID: WP_001302048.1
• Coordinates: 2545170..2545361 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG97_RS13400 (2540248)	2540248..2540466	-	219	WP_000683609.1	DUF4014 family protein	-
QMG97_RS13405 (2540594)	2540594..2540905	-	312	WP_001289673.1	hypothetical protein	-
QMG97_RS13410 (2540898)	2540898..2541125	-	228	WP_000699809.1	hypothetical protein	-
QMG97_RS13415 (2541122)	2541122..2541403	-	282	WP_000603384.1	DNA-binding protein	-
QMG97_RS13420 (2541436)	2541436..2542197	-	762	WP_000450872.1	DUF1627 domain-containing protein	-
QMG97_RS13425 (2542223)	2542223..2542964	-	742	Protein_2635	ATP-binding protein	-
QMG97_RS13430 (2542971)	2542971..2543933	-	963	WP_001444941.1	helix-turn-helix domain-containing protein	-
QMG97_RS13435 (2543956)	2543956..2544381	-	426	WP_000693943.1	toxin YdaT family protein	-
QMG97_RS13440 (2544378)	2544378..2544680	-	303	WP_000172738.1	transcriptional regulator	-
QMG97_RS13445 (2544778)	2544778..2545149	+	372	WP_001169687.1	hypothetical protein	-
QMG97_RS13450 (2545170)	2545170..2545361	+	192	WP_001302048.1	hypothetical protein	Antitoxin
QMG97_RS13455 (2545363)	2545363..2545641	+	279	WP_001448501.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QMG97_RS13460 (2545928)	2545928..2546080	+	153	WP_001427316.1	DUF1391 family protein	-
QMG97_RS13465 (2546501)	2546501..2546722	+	222	WP_000560218.1	killing protein KilR	-
QMG97_RS13470 (2546722)	2546722..2546892	+	171	WP_001427414.1	YdaE family protein	-
QMG97_RS13475 (2546966)	2546966..2547241	+	276	WP_001307773.1	hypothetical protein	-
QMG97_RS13480 (2547340)	2547340..2549991	+	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
QMG97_RS13485 (2549984)	2549984..2550388	+	405	Protein_2647	recombinase RecT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2495152..2559788	64636

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10691.27 Da
• Isoelectric Point: 5.5647

>T43449 WP_001448501.1 NZ_AP027197:2545363-2545641 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T43449 NZ_AP027197:2545363-2545641 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT43449 WP_001302048.1 NZ_AP027197:2545170-2545361 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT43449 NZ_AP027197:2545170-2545361 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A1Y2Y8G6

Antitoxin

• AlphaFold DB: A0A829CTP0