Detailed information of TA system TA043446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1999401..2000039
• Replicon: chromosome
• Accession: NZ_AP027197
• Organism: Escherichia coli strain 02E060

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QMG97_RS10570
• Protein ID: WP_000813794.1
• Coordinates: 1999401..1999577 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QMG97_RS10575
• Protein ID: WP_001270286.1
• Coordinates: 1999623..2000039 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG97_RS10550 (1995020)	1995020..1996234	-	1215	WP_001307190.1	BenE family transporter YdcO	-
QMG97_RS10555 (1996287)	1996287..1996823	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
QMG97_RS10560 (1996896)	1996896..1998857	+	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QMG97_RS10565 (1998949)	1998949..1999179	-	231	WP_000494244.1	YncJ family protein	-
QMG97_RS10570 (1999401)	1999401..1999577	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QMG97_RS10575 (1999623)	1999623..2000039	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QMG97_RS10580 (2000118)	2000118..2001523	+	1406	Protein_2072	PLP-dependent aminotransferase family protein	-
QMG97_RS10585 (2001768)	2001768..2002913	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
QMG97_RS10590 (2002931)	2002931..2003944	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
QMG97_RS10595 (2003945)	2003945..2004886	+	942	WP_001251332.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1993832..1994980	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43446 WP_000813794.1 NZ_AP027197:1999401-1999577 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43446 NZ_AP027197:1999401-1999577 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT43446 WP_001270286.1 NZ_AP027197:1999623-2000039 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT43446 NZ_AP027197:1999623-2000039 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB