Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9823..10466 Replicon plasmid pO26_10153_2
Accession NZ_AP027193
Organism Escherichia coli strain 10153

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag QMG89_RS30710 Protein ID WP_001044768.1
Coordinates 10050..10466 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag QMG89_RS30705 Protein ID WP_001261287.1
Coordinates 9823..10053 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG89_RS30675 (M10153_p2_04) 5688..7127 + 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -
QMG89_RS30680 7194..7337 + 144 Protein_4 transcriptional regulator -
QMG89_RS30685 (M10153_p2_05) 7341..7532 + 192 Protein_5 lipid II-degrading bacteriocin -
QMG89_RS30690 (M10153_p2_06) 7540..7893 - 354 WP_000864810.1 colicin M immunity protein -
QMG89_RS30695 (M10153_p2_07) 8066..8848 - 783 WP_001164205.1 site-specific integrase -
QMG89_RS30700 (M10153_p2_08) 8850..9263 - 414 WP_000465041.1 hypothetical protein -
QMG89_RS30705 (M10153_p2_09) 9823..10053 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QMG89_RS30710 (M10153_p2_10) 10050..10466 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
QMG89_RS30715 (M10153_p2_11) 10628..11173 - 546 WP_001165114.1 hypothetical protein -
QMG89_RS30720 (M10153_p2_12) 11207..14191 - 2985 WP_032275676.1 Tn3 family transposase -
QMG89_RS30725 (M10153_p2_13) 14352..14942 + 591 WP_000086536.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - hlyC / hlyA / hlyB / hlyD / toxB / espP 1..95214 95214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T43431 WP_001044768.1 NZ_AP027193:10050-10466 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T43431 NZ_AP027193:10050-10466 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT43431 WP_001261287.1 NZ_AP027193:9823-10053 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT43431 NZ_AP027193:9823-10053 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References