Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 66981..67582 Replicon plasmid pO26_10153_1
Accession NZ_AP027192
Organism Escherichia coli strain 10153

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag QMG89_RS30410 Protein ID WP_001216034.1
Coordinates 66981..67361 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag QMG89_RS30415 Protein ID WP_001190712.1
Coordinates 67361..67582 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG89_RS30380 62108..62338 - 231 Protein_63 ash family protein -
QMG89_RS30385 (M10153_p1_065) 62422..63906 - 1485 WP_000124153.1 hypothetical protein -
QMG89_RS30390 (M10153_p1_066) 63906..65099 - 1194 WP_000219603.1 terminase -
QMG89_RS30395 (M10153_p1_067) 65185..65607 - 423 WP_000405083.1 hypothetical protein -
QMG89_RS30400 (M10153_p1_068) 65726..66769 - 1044 WP_000648823.1 DUF968 domain-containing protein -
QMG89_RS30405 (M10153_p1_069) 66797..66976 - 180 WP_000113018.1 hypothetical protein -
QMG89_RS30410 (M10153_p1_070) 66981..67361 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
QMG89_RS30415 (M10153_p1_071) 67361..67582 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
QMG89_RS30420 (M10153_p1_072) 67655..68044 - 390 WP_000506726.1 S24 family peptidase -
QMG89_RS30425 (M10153_p1_073) 68219..68554 + 336 WP_001133672.1 hypothetical protein -
QMG89_RS30430 (M10153_p1_074) 68585..68800 + 216 WP_001048304.1 hypothetical protein -
QMG89_RS30435 (M10153_p1_075) 68861..69385 - 525 WP_000159954.1 toprim domain-containing protein -
QMG89_RS30440 (M10153_p1_076) 69382..69687 - 306 WP_000988220.1 hypothetical protein -
QMG89_RS30445 (M10153_p1_077) 69687..69971 - 285 WP_001142389.1 hypothetical protein -
QMG89_RS30450 (M10153_p1_078) 69955..70644 - 690 WP_000057456.1 hypothetical protein -
QMG89_RS30455 (M10153_p1_079) 70626..71000 - 375 WP_000988652.1 hypothetical protein -
QMG89_RS30460 (M10153_p1_080) 71007..71300 - 294 WP_000267997.1 hypothetical protein -
QMG89_RS30465 (M10153_p1_081) 71324..71605 - 282 WP_000002123.1 ASCH domain-containing protein -
QMG89_RS30470 (M10153_p1_082) 71605..71865 - 261 WP_103951243.1 eaa protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..100288 100288


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T43430 WP_001216034.1 NZ_AP027192:c67361-66981 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T43430 NZ_AP027192:c67361-66981 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT43430 WP_001190712.1 NZ_AP027192:c67582-67361 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT43430 NZ_AP027192:c67582-67361 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References