Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5455270..5455767 Replicon chromosome
Accession NZ_AP027191
Organism Escherichia coli strain 10153

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QMG89_RS28135 Protein ID WP_001173343.1
Coordinates 5455594..5455767 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QMG89_RS28130 Protein ID WP_000398619.1
Coordinates 5455270..5455566 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG89_RS28110 (5452001) 5452001..5452720 - 720 WP_000611288.1 two-component system response regulator DcuR -
QMG89_RS28115 (5452717) 5452717..5454348 - 1632 WP_001216487.1 sensor histidine kinase -
QMG89_RS28120 (5454529) 5454529..5454759 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QMG89_RS28125 (5454771) 5454771..5455043 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QMG89_RS28130 (5455270) 5455270..5455566 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QMG89_RS28135 (5455594) 5455594..5455767 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QMG89_RS28140 (5455886) 5455886..5457403 - 1518 WP_001295074.1 lysine--tRNA ligase -
QMG89_RS28145 (5457640) 5457640..5459097 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43425 WP_001173343.1 NZ_AP027191:5455594-5455767 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43425 NZ_AP027191:5455594-5455767 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43425 WP_000398619.1 NZ_AP027191:5455270-5455566 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43425 NZ_AP027191:5455270-5455566 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References