Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4081073..4081727 Replicon chromosome
Accession NZ_AP027191
Organism Escherichia coli strain 10153

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QMG89_RS21530 Protein ID WP_000244777.1
Coordinates 4081073..4081480 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QMG89_RS21535 Protein ID WP_000354046.1
Coordinates 4081461..4081727 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG89_RS21510 (4077030) 4077030..4078763 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
QMG89_RS21515 (4078769) 4078769..4079479 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QMG89_RS21520 (4079504) 4079504..4080400 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QMG89_RS21525 (4080512) 4080512..4081033 + 522 WP_001055874.1 flavodoxin FldB -
QMG89_RS21530 (4081073) 4081073..4081480 - 408 WP_000244777.1 protein YgfX Toxin
QMG89_RS21535 (4081461) 4081461..4081727 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QMG89_RS21540 (4081970) 4081970..4082950 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
QMG89_RS21545 (4083146) 4083146..4083805 - 660 WP_000250274.1 hemolysin III family protein -
QMG89_RS21550 (4083969) 4083969..4084280 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
QMG89_RS21555 (4084325) 4084325..4085758 + 1434 WP_001341859.1 6-phospho-beta-glucosidase BglA -
QMG89_RS21560 (4085815) 4085815..4086558 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T43422 WP_000244777.1 NZ_AP027191:c4081480-4081073 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43422 NZ_AP027191:c4081480-4081073 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43422 WP_000354046.1 NZ_AP027191:c4081727-4081461 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43422 NZ_AP027191:c4081727-4081461 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References