Detailed information of TA system TA043419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3543468..3544093
• Replicon: chromosome
• Accession: NZ_AP027191
• Organism: Escherichia coli strain 10153

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QMG89_RS18820
• Protein ID: WP_000911330.1
• Coordinates: 3543468..3543866 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QMG89_RS18825
• Protein ID: WP_000450524.1
• Coordinates: 3543866..3544093 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG89_RS18800 (3539346)	3539346..3539546	+	201	WP_000383836.1	YpfN family protein	-
QMG89_RS18805 (3539656)	3539656..3540354	-	699	WP_000679812.1	esterase	-
QMG89_RS18810 (3540428)	3540428..3542443	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
QMG89_RS18815 (3542458)	3542458..3543321	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QMG89_RS18820 (3543468)	3543468..3543866	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG89_RS18825 (3543866)	3543866..3544093	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QMG89_RS18830 (3544247)	3544247..3544960	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QMG89_RS18835 (3545173)	3545173..3546207	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QMG89_RS18840 (3546224)	3546224..3547102	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QMG89_RS18845 (3547248)	3547248..3547820	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QMG89_RS18850 (3547820)	3547820..3548290	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43419 WP_000911330.1 NZ_AP027191:c3543866-3543468 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43419 NZ_AP027191:c3543866-3543468 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43419 WP_000450524.1 NZ_AP027191:c3544093-3543866 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43419 NZ_AP027191:c3544093-3543866 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33