Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2672968..2673439 Replicon chromosome
Accession NZ_AP027191
Organism Escherichia coli strain 10153

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag QMG89_RS14370 Protein ID WP_001448501.1
Coordinates 2673161..2673439 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag QMG89_RS14365 Protein ID WP_001302048.1
Coordinates 2672968..2673159 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG89_RS14315 (2668046) 2668046..2668264 - 219 WP_000683609.1 DUF4014 family protein -
QMG89_RS14320 (2668392) 2668392..2668703 - 312 WP_001289673.1 hypothetical protein -
QMG89_RS14325 (2668696) 2668696..2668923 - 228 WP_000699809.1 hypothetical protein -
QMG89_RS14330 (2668920) 2668920..2669201 - 282 WP_000603384.1 DNA-binding protein -
QMG89_RS14335 (2669234) 2669234..2669995 - 762 WP_000450872.1 DUF1627 domain-containing protein -
QMG89_RS14340 (2670021) 2670021..2670762 - 742 Protein_2813 ATP-binding protein -
QMG89_RS14345 (2670769) 2670769..2671731 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
QMG89_RS14350 (2671754) 2671754..2672179 - 426 WP_000693943.1 toxin YdaT family protein -
QMG89_RS14355 (2672176) 2672176..2672478 - 303 WP_000172738.1 transcriptional regulator -
QMG89_RS14360 (2672576) 2672576..2672947 + 372 WP_001169687.1 hypothetical protein -
QMG89_RS14365 (2672968) 2672968..2673159 + 192 WP_001302048.1 hypothetical protein Antitoxin
QMG89_RS14370 (2673161) 2673161..2673439 + 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QMG89_RS14375 (2673726) 2673726..2673878 + 153 WP_001427316.1 DUF1391 family protein -
QMG89_RS14380 (2674299) 2674299..2674520 + 222 WP_000560218.1 killing protein KilR -
QMG89_RS14385 (2674520) 2674520..2674690 + 171 WP_001427414.1 YdaE family protein -
QMG89_RS14390 (2674764) 2674764..2675039 + 276 WP_001307773.1 hypothetical protein -
QMG89_RS14395 (2675138) 2675138..2677789 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 2615342..2709716 94374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T43408 WP_001448501.1 NZ_AP027191:2673161-2673439 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T43408 NZ_AP027191:2673161-2673439 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT43408 WP_001302048.1 NZ_AP027191:2672968-2673159 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT43408 NZ_AP027191:2672968-2673159 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References