Detailed information of TA system TA043406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2167110..2167748
• Replicon: chromosome
• Accession: NZ_AP027191
• Organism: Escherichia coli strain 10153

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QMG89_RS11735
• Protein ID: WP_000813794.1
• Coordinates: 2167110..2167286 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QMG89_RS11740
• Protein ID: WP_001270286.1
• Coordinates: 2167332..2167748 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG89_RS11715 (2162729)	2162729..2163943	-	1215	WP_001307190.1	BenE family transporter YdcO	-
QMG89_RS11720 (2163996)	2163996..2164532	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
QMG89_RS11725 (2164605)	2164605..2166566	+	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QMG89_RS11730 (2166658)	2166658..2166888	-	231	WP_000494244.1	YncJ family protein	-
QMG89_RS11735 (2167110)	2167110..2167286	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QMG89_RS11740 (2167332)	2167332..2167748	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QMG89_RS11745 (2167827)	2167827..2169232	+	1406	Protein_2300	PLP-dependent aminotransferase family protein	-
QMG89_RS11750 (2169477)	2169477..2170622	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
QMG89_RS11755 (2170640)	2170640..2171653	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
QMG89_RS11760 (2171654)	2171654..2172595	+	942	WP_001251332.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2161541..2162689	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43406 WP_000813794.1 NZ_AP027191:2167110-2167286 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43406 NZ_AP027191:2167110-2167286 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT43406 WP_001270286.1 NZ_AP027191:2167332-2167748 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT43406 NZ_AP027191:2167332-2167748 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB