Detailed information of TA system TA043395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 531989..532826
• Replicon: chromosome
• Accession: NZ_AP027191
• Organism: Escherichia coli strain 10153

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QMG89_RS02610
• Protein ID: WP_000227784.1
• Coordinates: 531989..532531 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QMG89_RS02615
• Protein ID: WP_001297137.1
• Coordinates: 532515..532826 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG89_RS02590 (527528)	527528..528439	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
QMG89_RS02595 (528607)	528607..529098	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QMG89_RS02600 (529226)	529226..530590	-	1365	WP_001000977.1	MFS transporter	-
QMG89_RS02605 (530998)	530998..531933	+	936	WP_001375746.1	tetratricopeptide repeat protein	-
QMG89_RS02610 (531989)	531989..532531	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QMG89_RS02615 (532515)	532515..532826	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QMG89_RS02620 (533011)	533011..533901	-	891	WP_000971336.1	heme o synthase	-
QMG89_RS02625 (533913)	533913..534242	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QMG89_RS02630 (534242)	534242..534856	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QMG89_RS02635 (534846)	534846..536837	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QMG89_RS02640 (536859)	536859..537806	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43395 WP_000227784.1 NZ_AP027191:c532531-531989 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43395 NZ_AP027191:c532531-531989 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43395 WP_001297137.1 NZ_AP027191:c532826-532515 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43395 NZ_AP027191:c532826-532515 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3