Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9823..10466 Replicon plasmid pO26_PV0838_1
Accession NZ_AP027189
Organism Escherichia coli strain PV0838

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag QMG68_RS29675 Protein ID WP_001044768.1
Coordinates 10050..10466 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag QMG68_RS29670 Protein ID WP_001261287.1
Coordinates 9823..10053 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG68_RS29640 (PV0838_p1_04) 5688..7127 + 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -
QMG68_RS29645 7194..7337 + 144 Protein_4 transcriptional regulator -
QMG68_RS29650 (PV0838_p1_05) 7341..7532 + 192 Protein_5 lipid II-degrading bacteriocin -
QMG68_RS29655 (PV0838_p1_06) 7540..7893 - 354 WP_000864810.1 colicin M immunity protein -
QMG68_RS29660 (PV0838_p1_07) 8066..8848 - 783 WP_001164205.1 site-specific integrase -
QMG68_RS29665 (PV0838_p1_08) 8850..9263 - 414 WP_000465041.1 hypothetical protein -
QMG68_RS29670 (PV0838_p1_09) 9823..10053 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QMG68_RS29675 (PV0838_p1_10) 10050..10466 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
QMG68_RS29680 (PV0838_p1_11) 10628..11173 - 546 WP_001165114.1 hypothetical protein -
QMG68_RS29685 (PV0838_p1_13) 11241..12397 + 1157 WP_085948186.1 IS3-like element IS600 family transposase -
QMG68_RS29690 (PV0838_p1_14) 12441..12527 - 87 Protein_13 IS3 family transposase -
QMG68_RS29695 (PV0838_p1_15) 12508..12984 - 477 WP_000422675.1 transposase -
QMG68_RS29700 13071..13185 + 115 Protein_15 IS3 family transposase -
QMG68_RS29705 13236..14746 - 1511 Protein_16 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - hlyC / hlyA / hlyB / hlyD / espP 1..70980 70980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T43393 WP_001044768.1 NZ_AP027189:10050-10466 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T43393 NZ_AP027189:10050-10466 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT43393 WP_001261287.1 NZ_AP027189:9823-10053 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT43393 NZ_AP027189:9823-10053 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References