Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5391669..5392166 Replicon chromosome
Accession NZ_AP027188
Organism Escherichia coli strain PV0838

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QMG68_RS27685 Protein ID WP_001173343.1
Coordinates 5391993..5392166 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QMG68_RS27680 Protein ID WP_000398619.1
Coordinates 5391669..5391965 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG68_RS27660 (5388400) 5388400..5389119 - 720 WP_000611288.1 two-component system response regulator DcuR -
QMG68_RS27665 (5389116) 5389116..5390747 - 1632 WP_001216487.1 sensor histidine kinase -
QMG68_RS27670 (5390928) 5390928..5391158 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QMG68_RS27675 (5391170) 5391170..5391442 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QMG68_RS27680 (5391669) 5391669..5391965 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QMG68_RS27685 (5391993) 5391993..5392166 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QMG68_RS27690 (5392285) 5392285..5393802 - 1518 WP_001295074.1 lysine--tRNA ligase -
QMG68_RS27695 (5394039) 5394039..5395496 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espG / escE / cesAB / escK / escL / escR / escS / escT / escU / etgA / cesD / escC / sepD / escJ / escI / sepZ/espZ / cesL / escV / escN / escO / sepQ/escQ / espH / cesF / map / tir / cesT / eae / escD / sepL / espA / espD / espB / cesD2 / escF / escG / espF / efa1 / nleE / nleB1 / espL2 5391669..5460141 68472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43388 WP_001173343.1 NZ_AP027188:5391993-5392166 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43388 NZ_AP027188:5391993-5392166 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43388 WP_000398619.1 NZ_AP027188:5391669-5391965 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43388 NZ_AP027188:5391669-5391965 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References