Detailed information of TA system TA043368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2045881..2046519
• Replicon: chromosome
• Accession: NZ_AP027188
• Organism: Escherichia coli strain PV0838

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QMG68_RS10925
• Protein ID: WP_000813794.1
• Coordinates: 2045881..2046057 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QMG68_RS10930
• Protein ID: WP_001270286.1
• Coordinates: 2046103..2046519 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG68_RS10905 (2041500)	2041500..2042714	-	1215	WP_001307190.1	BenE family transporter YdcO	-
QMG68_RS10910 (2042767)	2042767..2043303	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
QMG68_RS10915 (2043376)	2043376..2045337	+	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QMG68_RS10920 (2045429)	2045429..2045659	-	231	WP_000494244.1	YncJ family protein	-
QMG68_RS10925 (2045881)	2045881..2046057	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QMG68_RS10930 (2046103)	2046103..2046519	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QMG68_RS10935 (2046598)	2046598..2048003	+	1406	Protein_2138	PLP-dependent aminotransferase family protein	-
QMG68_RS10940 (2048248)	2048248..2049393	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
QMG68_RS10945 (2049411)	2049411..2050424	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
QMG68_RS10950 (2050425)	2050425..2051366	+	942	WP_001251332.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2040312..2041460	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43368 WP_000813794.1 NZ_AP027188:2045881-2046057 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43368 NZ_AP027188:2045881-2046057 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT43368 WP_001270286.1 NZ_AP027188:2046103-2046519 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT43368 NZ_AP027188:2046103-2046519 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB