Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5393571..5394068 Replicon chromosome
Accession NZ_AP027185
Organism Escherichia coli strain NIID080884

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QMG74_RS27470 Protein ID WP_001173343.1
Coordinates 5393895..5394068 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QMG74_RS27465 Protein ID WP_000398619.1
Coordinates 5393571..5393867 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG74_RS27445 (5390302) 5390302..5391021 - 720 WP_000611288.1 two-component system response regulator DcuR -
QMG74_RS27450 (5391018) 5391018..5392649 - 1632 WP_001216487.1 sensor histidine kinase -
QMG74_RS27455 (5392830) 5392830..5393060 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QMG74_RS27460 (5393072) 5393072..5393344 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QMG74_RS27465 (5393571) 5393571..5393867 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QMG74_RS27470 (5393895) 5393895..5394068 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QMG74_RS27475 (5394187) 5394187..5395704 - 1518 WP_001295074.1 lysine--tRNA ligase -
QMG74_RS27480 (5395941) 5395941..5397398 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43353 WP_001173343.1 NZ_AP027185:5393895-5394068 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43353 NZ_AP027185:5393895-5394068 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43353 WP_000398619.1 NZ_AP027185:5393571-5393867 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43353 NZ_AP027185:5393571-5393867 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References