Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4018271..4018925 Replicon chromosome
Accession NZ_AP027185
Organism Escherichia coli strain NIID080884

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QMG74_RS20865 Protein ID WP_000244777.1
Coordinates 4018271..4018678 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QMG74_RS20870 Protein ID WP_000354046.1
Coordinates 4018659..4018925 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG74_RS20845 (4014228) 4014228..4015961 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
QMG74_RS20850 (4015967) 4015967..4016677 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QMG74_RS20855 (4016702) 4016702..4017598 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QMG74_RS20860 (4017710) 4017710..4018231 + 522 WP_001055874.1 flavodoxin FldB -
QMG74_RS20865 (4018271) 4018271..4018678 - 408 WP_000244777.1 protein YgfX Toxin
QMG74_RS20870 (4018659) 4018659..4018925 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QMG74_RS20875 (4019168) 4019168..4020148 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
QMG74_RS20880 (4020344) 4020344..4021003 - 660 WP_000250274.1 hemolysin III family protein -
QMG74_RS20885 (4021167) 4021167..4021478 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
QMG74_RS20890 (4021523) 4021523..4022956 + 1434 WP_001341859.1 6-phospho-beta-glucosidase BglA -
QMG74_RS20895 (4023013) 4023013..4023756 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T43350 WP_000244777.1 NZ_AP027185:c4018678-4018271 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43350 NZ_AP027185:c4018678-4018271 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43350 WP_000354046.1 NZ_AP027185:c4018925-4018659 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43350 NZ_AP027185:c4018925-4018659 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References