Detailed information of TA system TA043347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3440235..3440860
• Replicon: chromosome
• Accession: NZ_AP027185
• Organism: Escherichia coli strain NIID080884

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QMG74_RS17840
• Protein ID: WP_000911330.1
• Coordinates: 3440235..3440633 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QMG74_RS17845
• Protein ID: WP_000450524.1
• Coordinates: 3440633..3440860 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG74_RS17820 (3436113)	3436113..3436313	+	201	WP_000383836.1	YpfN family protein	-
QMG74_RS17825 (3436423)	3436423..3437121	-	699	WP_000679812.1	esterase	-
QMG74_RS17830 (3437195)	3437195..3439210	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
QMG74_RS17835 (3439225)	3439225..3440088	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QMG74_RS17840 (3440235)	3440235..3440633	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG74_RS17845 (3440633)	3440633..3440860	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QMG74_RS17850 (3441014)	3441014..3441727	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QMG74_RS17855 (3441940)	3441940..3442974	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QMG74_RS17860 (3442991)	3442991..3443869	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QMG74_RS17865 (3444015)	3444015..3444587	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QMG74_RS17870 (3444587)	3444587..3445057	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43347 WP_000911330.1 NZ_AP027185:c3440633-3440235 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43347 NZ_AP027185:c3440633-3440235 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43347 WP_000450524.1 NZ_AP027185:c3440860-3440633 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43347 NZ_AP027185:c3440860-3440633 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33