Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2570173..2570644 Replicon chromosome
Accession NZ_AP027185
Organism Escherichia coli strain NIID080884

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag QMG74_RS13395 Protein ID WP_001448501.1
Coordinates 2570366..2570644 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag QMG74_RS13390 Protein ID WP_001302048.1
Coordinates 2570173..2570364 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG74_RS13340 (2565251) 2565251..2565469 - 219 WP_000683609.1 DUF4014 family protein -
QMG74_RS13345 (2565597) 2565597..2565908 - 312 WP_001289673.1 hypothetical protein -
QMG74_RS13350 (2565901) 2565901..2566128 - 228 WP_000699809.1 hypothetical protein -
QMG74_RS13355 (2566125) 2566125..2566406 - 282 WP_000603384.1 DNA-binding protein -
QMG74_RS13360 (2566439) 2566439..2567200 - 762 WP_000450872.1 DUF1627 domain-containing protein -
QMG74_RS13365 (2567226) 2567226..2567967 - 742 Protein_2620 ATP-binding protein -
QMG74_RS13370 (2567974) 2567974..2568936 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
QMG74_RS13375 (2568959) 2568959..2569384 - 426 WP_000693943.1 toxin YdaT family protein -
QMG74_RS13380 (2569381) 2569381..2569683 - 303 WP_000172738.1 transcriptional regulator -
QMG74_RS13385 (2569781) 2569781..2570152 + 372 WP_001169687.1 hypothetical protein -
QMG74_RS13390 (2570173) 2570173..2570364 + 192 WP_001302048.1 hypothetical protein Antitoxin
QMG74_RS13395 (2570366) 2570366..2570644 + 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QMG74_RS13400 (2570931) 2570931..2571083 + 153 WP_001427316.1 DUF1391 family protein -
QMG74_RS13405 (2571504) 2571504..2571725 + 222 WP_000560218.1 killing protein KilR -
QMG74_RS13410 (2571725) 2571725..2571895 + 171 WP_001427414.1 YdaE family protein -
QMG74_RS13415 (2571969) 2571969..2572244 + 276 WP_001307773.1 hypothetical protein -
QMG74_RS13420 (2572343) 2572343..2574994 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 2512547..2618211 105664


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T43336 WP_001448501.1 NZ_AP027185:2570366-2570644 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T43336 NZ_AP027185:2570366-2570644 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT43336 WP_001302048.1 NZ_AP027185:2570173-2570364 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT43336 NZ_AP027185:2570173-2570364 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References