Detailed information of TA system TA043334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2014404..2015042
• Replicon: chromosome
• Accession: NZ_AP027185
• Organism: Escherichia coli strain NIID080884

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QMG74_RS10500
• Protein ID: WP_000813794.1
• Coordinates: 2014404..2014580 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QMG74_RS10505
• Protein ID: WP_001270286.1
• Coordinates: 2014626..2015042 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG74_RS10480 (2010023)	2010023..2011237	-	1215	WP_001307190.1	BenE family transporter YdcO	-
QMG74_RS10485 (2011290)	2011290..2011826	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
QMG74_RS10490 (2011899)	2011899..2013860	+	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QMG74_RS10495 (2013952)	2013952..2014182	-	231	WP_000494244.1	YncJ family protein	-
QMG74_RS10500 (2014404)	2014404..2014580	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QMG74_RS10505 (2014626)	2014626..2015042	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QMG74_RS10510 (2015121)	2015121..2016526	+	1406	Protein_2058	PLP-dependent aminotransferase family protein	-
QMG74_RS10515 (2016771)	2016771..2017916	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
QMG74_RS10520 (2017934)	2017934..2018947	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
QMG74_RS10525 (2018948)	2018948..2019889	+	942	WP_001251332.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2008835..2009983	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43334 WP_000813794.1 NZ_AP027185:2014404-2014580 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43334 NZ_AP027185:2014404-2014580 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT43334 WP_001270286.1 NZ_AP027185:2014626-2015042 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT43334 NZ_AP027185:2014626-2015042 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB