Detailed information of TA system TA043327

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 391660..392461
• Replicon: chromosome
• Accession: NZ_AP027185
• Organism: Escherichia coli strain NIID080884

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: QMG74_RS01895
• Protein ID: WP_001094440.1
• Coordinates: 392084..392461 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: QMG74_RS01890
• Protein ID: WP_024166067.1
• Coordinates: 391660..392037 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG74_RS01855 (387819)	387819..388502	+	684	WP_000775504.1	hypothetical protein	-
QMG74_RS01860 (388518)	388518..388928	+	411	WP_000846706.1	hypothetical protein	-
QMG74_RS01865 (389149)	389149..389967	+	819	WP_001234621.1	DUF932 domain-containing protein	-
QMG74_RS01870 (389967)	389967..390212	+	246	WP_000680583.1	hypothetical protein	-
QMG74_RS01875 (390306)	390306..390785	+	480	WP_001449312.1	antirestriction protein	-
QMG74_RS01880 (390801)	390801..391277	+	477	WP_001367144.1	RadC family protein	-
QMG74_RS01885 (391364)	391364..391585	+	222	WP_000692350.1	DUF987 domain-containing protein	-
QMG74_RS01890 (391660)	391660..392037	+	378	WP_024166067.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QMG74_RS01895 (392084)	392084..392461	+	378	WP_001094440.1	TA system toxin CbtA family protein	Toxin
QMG74_RS01900 (392458)	392458..392946	+	489	WP_000761703.1	DUF5983 family protein	-
QMG74_RS01905 (392958)	392958..393155	+	198	WP_000839232.1	DUF957 domain-containing protein	-
QMG74_RS01910 (393369)	393369..394388	+	1020	WP_000875212.1	IS110 family transposase	-
QMG74_RS01915 (394671)	394671..395519	+	849	WP_001280444.1	DUF4942 domain-containing protein	-
QMG74_RS01920 (395612)	395612..396931	-	1320	WP_000144685.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(B) / aph(3'')-Ib / aph(6)-Id / sul2	-	347030..415879	68849
	-	flank	IS/Tn	-	-	393369..394388	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13961.87 Da
• Isoelectric Point: 7.3225

>T43327 WP_001094440.1 NZ_AP027185:392084-392461 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T43327 NZ_AP027185:392084-392461 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGTCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCTTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13676.53 Da
• Isoelectric Point: 5.9505

>AT43327 WP_024166067.1 NZ_AP027185:391660-392037 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT43327 NZ_AP027185:391660-392037 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA