Detailed information of TA system TA043325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9823..10466
• Replicon: plasmid pO26_98E11_2
• Accession: NZ_AP027183
• Organism: Escherichia coli strain 98E11

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: QMG82_RS30250
• Protein ID: WP_001044768.1
• Coordinates: 10050..10466 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: QMG82_RS30245
• Protein ID: WP_001261287.1
• Coordinates: 9823..10053 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG82_RS30215 (F98E11_p2_04)	5688..7127	+	1440	WP_001213545.1	enterohemolysin T1SS ABC transporter subunit EhxD	-
QMG82_RS30220	7194..7337	+	144	Protein_4	transcriptional regulator	-
QMG82_RS30225 (F98E11_p2_05)	7341..7532	+	192	Protein_5	lipid II-degrading bacteriocin	-
QMG82_RS30230 (F98E11_p2_06)	7540..7893	-	354	WP_000864810.1	colicin M immunity protein	-
QMG82_RS30235 (F98E11_p2_07)	8066..8848	-	783	WP_001164205.1	site-specific integrase	-
QMG82_RS30240 (F98E11_p2_08)	8850..9263	-	414	WP_000465041.1	hypothetical protein	-
QMG82_RS30245 (F98E11_p2_09)	9823..10053	+	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
QMG82_RS30250 (F98E11_p2_10)	10050..10466	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG82_RS30255 (F98E11_p2_11)	10628..11173	-	546	WP_001165114.1	hypothetical protein	-
QMG82_RS30260 (F98E11_p2_13)	11241..12397	+	1157	WP_085948186.1	IS3-like element IS600 family transposase	-
QMG82_RS30265 (F98E11_p2_14)	12555..13415	-	861	WP_000704534.1	alpha/beta hydrolase	-
QMG82_RS30270 (F98E11_p2_15)	13543..13899	+	357	Protein_14	tyrosine-type recombinase/integrase	-
QMG82_RS30275 (F98E11_p2_16)	13983..14657	+	675	WP_001341423.1	IS66-like element accessory protein TnpA	-
QMG82_RS30280 (F98E11_p2_17)	14654..15001	+	348	WP_000631725.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB / espP	1..92173	92173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T43325 WP_001044768.1 NZ_AP027183:10050-10466 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T43325 NZ_AP027183:10050-10466 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT43325 WP_001261287.1 NZ_AP027183:9823-10053 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT43325 NZ_AP027183:9823-10053 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4