Detailed information of TA system TA043313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3398424..3399049
• Replicon: chromosome
• Accession: NZ_AP027181
• Organism: Escherichia coli strain 98E11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QMG82_RS17790
• Protein ID: WP_000911330.1
• Coordinates: 3398424..3398822 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QMG82_RS17795
• Protein ID: WP_000450524.1
• Coordinates: 3398822..3399049 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG82_RS17770 (3394302)	3394302..3394502	+	201	WP_000383836.1	YpfN family protein	-
QMG82_RS17775 (3394612)	3394612..3395310	-	699	WP_000679812.1	esterase	-
QMG82_RS17780 (3395384)	3395384..3397399	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
QMG82_RS17785 (3397414)	3397414..3398277	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QMG82_RS17790 (3398424)	3398424..3398822	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG82_RS17795 (3398822)	3398822..3399049	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QMG82_RS17800 (3399203)	3399203..3399916	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QMG82_RS17805 (3400129)	3400129..3401163	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QMG82_RS17810 (3401180)	3401180..3402058	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QMG82_RS17815 (3402204)	3402204..3402776	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QMG82_RS17820 (3402776)	3402776..3403246	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43313 WP_000911330.1 NZ_AP027181:c3398822-3398424 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43313 NZ_AP027181:c3398822-3398424 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43313 WP_000450524.1 NZ_AP027181:c3399049-3398822 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43313 NZ_AP027181:c3399049-3398822 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33