Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2528838..2529309 Replicon chromosome
Accession NZ_AP027181
Organism Escherichia coli strain 98E11

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag QMG82_RS13355 Protein ID WP_001448501.1
Coordinates 2529031..2529309 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag QMG82_RS13350 Protein ID WP_001302048.1
Coordinates 2528838..2529029 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG82_RS13300 (2523916) 2523916..2524134 - 219 WP_000683609.1 DUF4014 family protein -
QMG82_RS13305 (2524262) 2524262..2524573 - 312 WP_001289673.1 hypothetical protein -
QMG82_RS13310 (2524566) 2524566..2524793 - 228 WP_000699809.1 hypothetical protein -
QMG82_RS13315 (2524790) 2524790..2525071 - 282 WP_000603384.1 DNA-binding protein -
QMG82_RS13320 (2525104) 2525104..2525865 - 762 WP_000450872.1 DUF1627 domain-containing protein -
QMG82_RS13325 (2525891) 2525891..2526632 - 742 Protein_2616 ATP-binding protein -
QMG82_RS13330 (2526639) 2526639..2527601 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
QMG82_RS13335 (2527624) 2527624..2528049 - 426 WP_000693943.1 toxin YdaT family protein -
QMG82_RS13340 (2528046) 2528046..2528348 - 303 WP_000172738.1 transcriptional regulator -
QMG82_RS13345 (2528446) 2528446..2528817 + 372 WP_001169687.1 hypothetical protein -
QMG82_RS13350 (2528838) 2528838..2529029 + 192 WP_001302048.1 hypothetical protein Antitoxin
QMG82_RS13355 (2529031) 2529031..2529309 + 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QMG82_RS13360 (2529596) 2529596..2529748 + 153 WP_001427316.1 DUF1391 family protein -
QMG82_RS13365 (2530169) 2530169..2530390 + 222 WP_000560218.1 killing protein KilR -
QMG82_RS13370 (2530390) 2530390..2530560 + 171 WP_001427414.1 YdaE family protein -
QMG82_RS13375 (2530634) 2530634..2530909 + 276 WP_001307773.1 hypothetical protein -
QMG82_RS13380 (2531008) 2531008..2533659 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 2469945..2544097 74152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T43302 WP_001448501.1 NZ_AP027181:2529031-2529309 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T43302 NZ_AP027181:2529031-2529309 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT43302 WP_001302048.1 NZ_AP027181:2528838-2529029 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT43302 NZ_AP027181:2528838-2529029 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References