Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-Phd |
| Location | 63814..64415 | Replicon | plasmid pO26_EH2252_2 |
| Accession | NZ_AP027178 | ||
| Organism | Escherichia coli strain EH2252 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | U9YA20 |
| Locus tag | QMG71_RS30690 | Protein ID | WP_001216045.1 |
| Coordinates | 63814..64194 (-) | Length | 127 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | U9YQH9 |
| Locus tag | QMG71_RS30695 | Protein ID | WP_001190712.1 |
| Coordinates | 64194..64415 (-) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QMG71_RS30660 | 58941..59171 | - | 231 | Protein_61 | ash family protein | - |
| QMG71_RS30665 (EH2252_p2_060) | 59255..60739 | - | 1485 | WP_000124159.1 | hypothetical protein | - |
| QMG71_RS30670 (EH2252_p2_061) | 60739..61932 | - | 1194 | WP_023442315.1 | hypothetical protein | - |
| QMG71_RS30675 (EH2252_p2_062) | 62018..62470 | - | 453 | WP_001326849.1 | late promoter-activating protein | - |
| QMG71_RS30680 (EH2252_p2_063) | 62559..63602 | - | 1044 | WP_000648833.1 | DUF968 domain-containing protein | - |
| QMG71_RS30685 (EH2252_p2_064) | 63630..63809 | - | 180 | WP_000113018.1 | hypothetical protein | - |
| QMG71_RS30690 (EH2252_p2_065) | 63814..64194 | - | 381 | WP_001216045.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| QMG71_RS30695 (EH2252_p2_066) | 64194..64415 | - | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| QMG71_RS30700 (EH2252_p2_067) | 64488..64877 | - | 390 | WP_000506730.1 | S24 family peptidase | - |
| QMG71_RS30705 (EH2252_p2_068) | 64999..65277 | - | 279 | WP_136748066.1 | hypothetical protein | - |
| QMG71_RS30710 (EH2252_p2_070) | 65550..66230 | - | 681 | WP_044862031.1 | hypothetical protein | - |
| QMG71_RS30715 (EH2252_p2_071) | 66212..66586 | - | 375 | WP_000988656.1 | hypothetical protein | - |
| QMG71_RS30720 (EH2252_p2_072) | 66593..66886 | - | 294 | WP_000269002.1 | hypothetical protein | - |
| QMG71_RS30725 (EH2252_p2_074) | 67065..67298 | - | 234 | WP_000517421.1 | hypothetical protein | - |
| QMG71_RS30730 (EH2252_p2_075) | 67381..68079 | - | 699 | WP_044862032.1 | DUF551 domain-containing protein | - |
| QMG71_RS30735 (EH2252_p2_076) | 68280..68492 | - | 213 | WP_000063625.1 | hypothetical protein | - |
| QMG71_RS30740 (EH2252_p2_077) | 68541..68897 | - | 357 | WP_000403779.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..96161 | 96161 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 127 a.a. Molecular weight: 13588.29 Da Isoelectric Point: 5.1514
>T43286 WP_001216045.1 NZ_AP027178:c64194-63814 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
Download Length: 381 bp
>T43286 NZ_AP027178:c64194-63814 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG
Antitoxin
Download Length: 74 a.a. Molecular weight: 8133.10 Da Isoelectric Point: 4.7708
>AT43286 WP_001190712.1 NZ_AP027178:c64415-64194 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Download Length: 222 bp
>AT43286 NZ_AP027178:c64415-64194 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CLP7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CJB6 |