Detailed information of TA system TA043281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 71120..71667
• Replicon: plasmid pO26_EH2252_1
• Accession: NZ_AP027177
• Organism: Escherichia coli strain EH2252

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: QMG71_RS29870
• Protein ID: WP_097309154.1
• Coordinates: 71120..71398 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: QMG71_RS29875
• Protein ID: WP_000079941.1
• Coordinates: 71398..71667 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG71_RS29845 (67385)	67385..68419	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
QMG71_RS29850 (68600)	68600..68900	+	301	Protein_72	hypothetical protein	-
QMG71_RS29855 (68978)	68978..69325	-	348	WP_000142437.1	DUF6404 family protein	-
QMG71_RS29860 (69627)	69627..70109	-	483	WP_001311056.1	hypothetical protein	-
QMG71_RS29865 (70226)	70226..71074	-	849	WP_001058005.1	3'-5' exonuclease	-
QMG71_RS29870 (71120)	71120..71398	-	279	WP_097309154.1	type II toxin-antitoxin system toxin YacB	Toxin
QMG71_RS29875 (71398)	71398..71667	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
QMG71_RS29880 (72189)	72189..73425	+	1237	Protein_78	IS3 family transposase	-
QMG71_RS29885 (73622)	73622..74896	+	1275	WP_000489607.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) / sitABCD	iroB / iroC / iroD / iroE / iroN	1..147858	147858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10799.42 Da
• Isoelectric Point: 8.4615

>T43281 WP_097309154.1 NZ_AP027177:c71398-71120 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTVQKWP

Nucleotide

• Length: 279 bp

>T43281 NZ_AP027177:c71398-71120 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATAGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT43281 WP_000079941.1 NZ_AP027177:c71667-71398 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT43281 NZ_AP027177:c71667-71398 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5