Detailed information of TA system TA043280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 36380..37023
• Replicon: plasmid pO26_EH2252_1
• Accession: NZ_AP027177
• Organism: Escherichia coli strain EH2252

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: QMG71_RS29715
• Protein ID: WP_001044768.1
• Coordinates: 36380..36796 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: QMG71_RS29720
• Protein ID: WP_001261287.1
• Coordinates: 36793..37023 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG71_RS29685 (32551)	32551..33201	+	651	WP_000164043.1	tetracycline resistance transcriptional repressor TetR(A)	-
QMG71_RS29690 (33233)	33233..33421	-	189	WP_001191969.1	hypothetical protein	-
QMG71_RS29695 (33428)	33428..33994	-	567	WP_003159185.1	recombinase family protein	-
QMG71_RS29700 (34123)	34123..34371	-	249	WP_011191340.1	type II toxin-antitoxin system YafQ family toxin	-
QMG71_RS29705 (34630)	34630..35334	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
QMG71_RS29710 (35388)	35388..36218	+	831	Protein_44	AAA family ATPase	-
QMG71_RS29715 (36380)	36380..36796	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG71_RS29720 (36793)	36793..37023	-	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
QMG71_RS29725 (37319)	37319..37609	+	291	WP_000111771.1	hypothetical protein	-
QMG71_RS29730 (37599)	37599..38498	+	900	WP_000963206.1	nucleotide-binding protein	-
QMG71_RS29735 (38548)	38548..40773	-	2226	WP_000698737.1	P-loop NTPase fold protein	-
QMG71_RS29740 (40775)	40775..41863	-	1089	WP_000952217.1	transcriptional repressor PifC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) / sitABCD	iroB / iroC / iroD / iroE / iroN	1..147858	147858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T43280 WP_001044768.1 NZ_AP027177:c36796-36380 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T43280 NZ_AP027177:c36796-36380 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT43280 WP_001261287.1 NZ_AP027177:c37023-36793 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT43280 NZ_AP027177:c37023-36793 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4