Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 5553057..5553652 Replicon chromosome
Accession NZ_AP027176
Organism Escherichia coli strain EH2252

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag QMG71_RS28380 Protein ID WP_000239579.1
Coordinates 5553302..5553652 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag QMG71_RS28375 Protein ID WP_001223208.1
Coordinates 5553057..5553308 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG71_RS28365 (5548722) 5548722..5552501 + 3780 WP_000060926.1 autotransporter assembly complex protein TamB -
QMG71_RS28370 (5552504) 5552504..5552845 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
QMG71_RS28375 (5553057) 5553057..5553308 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
QMG71_RS28380 (5553302) 5553302..5553652 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
QMG71_RS28385 (5553732) 5553732..5554262 - 531 WP_000055075.1 inorganic diphosphatase -
QMG71_RS28390 (5554572) 5554572..5555528 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
QMG71_RS28395 (5555668) 5555668..5557170 + 1503 WP_000205813.1 sugar ABC transporter ATP-binding protein -
QMG71_RS28400 (5557184) 5557184..5558206 + 1023 WP_141078451.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T43276 WP_000239579.1 NZ_AP027176:5553302-5553652 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T43276 NZ_AP027176:5553302-5553652 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT43276 WP_001223208.1 NZ_AP027176:5553057-5553308 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT43276 NZ_AP027176:5553057-5553308 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References