Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5393858..5394355 Replicon chromosome
Accession NZ_AP027176
Organism Escherichia coli strain EH2252

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QMG71_RS27570 Protein ID WP_001173343.1
Coordinates 5394182..5394355 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QMG71_RS27565 Protein ID WP_000398619.1
Coordinates 5393858..5394154 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG71_RS27545 (5390589) 5390589..5391308 - 720 WP_000611288.1 two-component system response regulator DcuR -
QMG71_RS27550 (5391305) 5391305..5392936 - 1632 WP_001216487.1 sensor histidine kinase -
QMG71_RS27555 (5393117) 5393117..5393347 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QMG71_RS27560 (5393359) 5393359..5393631 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QMG71_RS27565 (5393858) 5393858..5394154 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QMG71_RS27570 (5394182) 5394182..5394355 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QMG71_RS27575 (5394474) 5394474..5395991 - 1518 WP_001295074.1 lysine--tRNA ligase -
QMG71_RS27580 (5396228) 5396228..5397685 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43275 WP_001173343.1 NZ_AP027176:5394182-5394355 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43275 NZ_AP027176:5394182-5394355 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43275 WP_000398619.1 NZ_AP027176:5393858-5394154 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43275 NZ_AP027176:5393858-5394154 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References