Detailed information of TA system TA043269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3471156..3471781
• Replicon: chromosome
• Accession: NZ_AP027176
• Organism: Escherichia coli strain EH2252

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QMG71_RS18170
• Protein ID: WP_000911330.1
• Coordinates: 3471156..3471554 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QMG71_RS18175
• Protein ID: WP_000450524.1
• Coordinates: 3471554..3471781 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG71_RS18150 (3467034)	3467034..3467234	+	201	WP_000383836.1	YpfN family protein	-
QMG71_RS18155 (3467344)	3467344..3468042	-	699	WP_000679812.1	esterase	-
QMG71_RS18160 (3468116)	3468116..3470131	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
QMG71_RS18165 (3470146)	3470146..3471009	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
QMG71_RS18170 (3471156)	3471156..3471554	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QMG71_RS18175 (3471554)	3471554..3471781	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QMG71_RS18180 (3471935)	3471935..3472648	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QMG71_RS18185 (3472861)	3472861..3473895	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
QMG71_RS18190 (3473912)	3473912..3474790	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QMG71_RS18195 (3474936)	3474936..3475508	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QMG71_RS18200 (3475508)	3475508..3475978	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43269 WP_000911330.1 NZ_AP027176:c3471554-3471156 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43269 NZ_AP027176:c3471554-3471156 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43269 WP_000450524.1 NZ_AP027176:c3471781-3471554 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43269 NZ_AP027176:c3471781-3471554 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33