Detailed information of TA system TA043255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2055911..2056549
• Replicon: chromosome
• Accession: NZ_AP027176
• Organism: Escherichia coli strain EH2252

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QMG71_RS10870
• Protein ID: WP_000813794.1
• Coordinates: 2055911..2056087 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QMG71_RS10875
• Protein ID: WP_001270286.1
• Coordinates: 2056133..2056549 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG71_RS10850 (2051530)	2051530..2052744	-	1215	WP_001307190.1	BenE family transporter YdcO	-
QMG71_RS10855 (2052797)	2052797..2053333	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
QMG71_RS10860 (2053406)	2053406..2055367	+	1962	WP_044862087.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QMG71_RS10865 (2055459)	2055459..2055689	-	231	WP_000494244.1	YncJ family protein	-
QMG71_RS10870 (2055911)	2055911..2056087	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QMG71_RS10875 (2056133)	2056133..2056549	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QMG71_RS10880 (2056628)	2056628..2058033	+	1406	Protein_2133	PLP-dependent aminotransferase family protein	-
QMG71_RS10885 (2058278)	2058278..2059423	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
QMG71_RS10890 (2059441)	2059441..2060454	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
QMG71_RS10895 (2060455)	2060455..2061396	+	942	WP_001251332.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2050342..2051490	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43255 WP_000813794.1 NZ_AP027176:2055911-2056087 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43255 NZ_AP027176:2055911-2056087 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT43255 WP_001270286.1 NZ_AP027176:2056133-2056549 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT43255 NZ_AP027176:2056133-2056549 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB