Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 517235..517853 Replicon chromosome
Accession NZ_AP027176
Organism Escherichia coli strain EH2252

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QMG71_RS02445 Protein ID WP_001291435.1
Coordinates 517235..517453 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QMG71_RS02450 Protein ID WP_000344800.1
Coordinates 517479..517853 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG71_RS02410 (512525) 512525..513097 + 573 WP_000779826.1 YbaY family lipoprotein -
QMG71_RS02415 (513128) 513128..513439 - 312 WP_000409911.1 MGMT family protein -
QMG71_RS02425 (513817) 513817..514170 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QMG71_RS02430 (514212) 514212..515762 - 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QMG71_RS02435 (515926) 515926..516396 - 471 WP_000136192.1 YlaC family protein -
QMG71_RS02440 (516512) 516512..517063 - 552 WP_000102564.1 maltose O-acetyltransferase -
QMG71_RS02445 (517235) 517235..517453 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QMG71_RS02450 (517479) 517479..517853 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QMG71_RS02455 (518399) 518399..521548 - 3150 WP_001132475.1 efflux RND transporter permease AcrB -
QMG71_RS02460 (521571) 521571..522764 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T43245 WP_001291435.1 NZ_AP027176:c517453-517235 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T43245 NZ_AP027176:c517453-517235 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT43245 WP_000344800.1 NZ_AP027176:c517853-517479 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT43245 NZ_AP027176:c517853-517479 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References