Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18876..19519 Replicon plasmid pO26_20.1_1
Accession NZ_AP027171
Organism Escherichia coli strain 20.1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag QMG87_RS28995 Protein ID WP_001044768.1
Coordinates 18876..19292 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag QMG87_RS29000 Protein ID WP_001261287.1
Coordinates 19289..19519 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG87_RS28965 (13995) 13995..14798 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
QMG87_RS28970 (14859) 14859..15674 - 816 WP_001043265.1 sulfonamide-resistant dihydropteroate synthase Sul2 -
QMG87_RS28975 (15982) 15982..16833 - 852 WP_000240536.1 replication protein C, IncQ-type -
QMG87_RS28980 (16820) 16820..17539 - 720 Protein_23 helicase RepA family protein -
QMG87_RS28985 (17589) 17589..18293 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
QMG87_RS28990 (18361) 18361..18714 + 354 WP_001427337.1 hypothetical protein -
QMG87_RS28995 (18876) 18876..19292 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
QMG87_RS29000 (19289) 19289..19519 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QMG87_RS29005 (19815) 19815..20105 + 291 WP_000111771.1 hypothetical protein -
QMG87_RS29010 (20095) 20095..20994 + 900 WP_000963206.1 nucleotide-binding protein -
QMG87_RS29015 (21044) 21044..23269 - 2226 WP_000698737.1 P-loop NTPase fold protein -
QMG87_RS29020 (23271) 23271..24359 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD iroN / iroE / iroD / iroC / iroB 1..129563 129563


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T43236 WP_001044768.1 NZ_AP027171:c19292-18876 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T43236 NZ_AP027171:c19292-18876 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT43236 WP_001261287.1 NZ_AP027171:c19519-19289 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT43236 NZ_AP027171:c19519-19289 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References