Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 5452280..5452875 Replicon chromosome
Accession NZ_AP027170
Organism Escherichia coli strain 20.1

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag QMG87_RS27755 Protein ID WP_000239579.1
Coordinates 5452525..5452875 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag QMG87_RS27750 Protein ID WP_001223208.1
Coordinates 5452280..5452531 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG87_RS27740 (5447945) 5447945..5451724 + 3780 WP_000060926.1 autotransporter assembly complex protein TamB -
QMG87_RS27745 (5451727) 5451727..5452068 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
QMG87_RS27750 (5452280) 5452280..5452531 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
QMG87_RS27755 (5452525) 5452525..5452875 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
QMG87_RS27760 (5452955) 5452955..5453485 - 531 WP_000055075.1 inorganic diphosphatase -
QMG87_RS27765 (5453795) 5453795..5454751 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
QMG87_RS27770 (5454891) 5454891..5456393 + 1503 WP_000205813.1 sugar ABC transporter ATP-binding protein -
QMG87_RS27775 (5456407) 5456407..5457429 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T43232 WP_000239579.1 NZ_AP027170:5452525-5452875 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T43232 NZ_AP027170:5452525-5452875 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT43232 WP_001223208.1 NZ_AP027170:5452280-5452531 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT43232 NZ_AP027170:5452280-5452531 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References